Bio-Imaging SIMS: How did we get here? What can we do? Where can we go?

John Fletcher

University of Gothenburg, Sweden

Spatial lipidomics in the larval brain using OrbiSIMS imaging

Yuhong Jin¹, Clare Newell¹, Ian Gilmore², Alex Gould¹

1: Francis Crick Institute, 1 Midland Road, London, NW1 1AT, United Kingdom; 2: National Physical Laboratory, Hampton Road, Teddington, TW11 0LW, United Kingdom

Water matrix secondary ion yield enhancement exemplified by tissue images in cryo-OrbiSIMS

Anya C. S. Eyres¹, Clare Newell^1,2, Junting Zhang¹, Gustavo F. Trindade¹, Alex Gould², Ian S. Gilmore¹

1: NiCE-MSI, National Physical Laboratory, Hampton Road, Teddington, Middlesex, TW11 0LW; 2: Physiology and Metabolism Laboratory, The Francis Crick Institute, 1 Midland Way, London, NW1 1AT

Understanding the Percutaneous Uptake of Agrochemicals using Mass Spectrometry Imaging; 3D OrbiSIMS

Mohammed Haroon Khan, David Scurr

University of Nottingham, United Kingdom

Mechanical Properties Measurement using Water GCIB for Biological Materials

Naoko Sano, Kate McHardy, Paul Blenkinsopp

Ionoptika Ltd, United Kingdom

The complex and mysterious analysis of glycosaminoglycans using SIMS

Andrew Leslie Hook

University of Nottingham, United Kingdom

Multi-dimensional tracking of fungicide in plant systems with mass spectrometry imaging

Akhila Ajith¹, Phillip J Milnes², Giles N Johnson³, Nicholas P Lockyer¹

1: Department of Chemistry, Photon Science Institute, University of Manchester, Manchester M13 9PL, UK; 2: Syngenta, Jeolott’s Hill International Research Centre, Bracknell RG42 6EY, UK; 3: Department of Earth and Environmental Sciences, University of Manchester, Manchester M13 9PY, UK

Application of ToF-SIMS and XPS to the analysis of Alum adjuvant for vaccine development

Giuditta Guerrini¹, Jorge Banus-Ciscar², Francesco Fumagalli², Luigi Calzolai², Giacomo Ceccone²

1: Università di Siena Dipartimento di Biotecnologie MedicheLAMMB (Biotechnology and Molecular Microbiology Lab.; 2: European Commission - Joint Research Centre

Deciphering compositional and structural features of mesenchymal stromal cell-derived extracellular matrices by discriminant principal component analysis of ToF-SIMS spectra

Ralf Zimmermann¹, Mirko Nitschke¹, Valentina Magno¹, Uwe Freudenberg¹, Katja Sockel², Friedrich Stölzel³, Manja Wobus², Uwe Platzbecker⁴, Carsten Werner¹

1: Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Dresden, Germany; 2: Medical Clinic and Policlinic I, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany; 3: Division of Stem Cell Transplantation and Cellular Immunotherapies, Department of Internal Medicine II, University Hospital Schleswig-Holstein, Kiel, Germany; 4: Hematology and Cellular Therapy, University Hospital Leipzig, Leipzig, Germany

Application of TOF-SIMS in Component Characterization of Complex Mixture Systems

Zhanping Li

Tsinghua University, China, People's Republic of

Native State Physicochemical Characterisation of Drug Delivery Hydrogels using Cryo-OrbiSIMS and SEM

Julie A Watts, David J Scurr

University of Nottingham, United Kingdom

Time-of-Flight surface spectrometry as a powerful ally to explore the molecular composition of complex biological systems

Xavier Delvaux, Abigail Wattiez, Yves Poumay, Laurent Houssiau

University of Namur, Belgium

Microvolume expansion using large argon clusters to enhance molecular ion signals and matrix selection in ToF-SIMS

Benjamin Tomasetti, Clément Lauzin, Arnaud Delcorte

Institute of Condensed Matter and Nanosciences - UClouvain, Belgium

The evolution and future of SIMS in the microelectronic industry

Alexis Franquet¹, Valentina Spampinato^1,2

1: IMEC, Belgium; 2: Università degli Studi di Catania, Italy

OrbiSIMS Investigations of Engine Deposits from Real-World Systems and the Laboratory

Joseph Lamb^1,2, Max Edney^1,2, Matteo Spanu², Jim Barker¹, Colin Snape², David Scurr², Emily Smith², Craig Stoppiello^2,3, Jacqueline Reid¹, Edward Wilmot¹, Morgan Alexander²

1: Innospec; 2: University of Nottingham; 3: University of Queensland

Correlative characterization of conversion coatings by using time-of-flight secondary ion mass spectrometry and salt spray tests

Damien Cossement¹, Emeline Lachery¹, Arno Schut², Patrick Karpe², Sylvain Desprez¹, Mireille Poelman¹

1: Materia Nova, 3 Avenue Nicolas Copernic, 7000 Mons, Belgium; 2: Axcentive, 593 Chemin de Champouse 13320 Bouc-Bel-Air, France

Microelectronic applications of in-situ FIB sectioning in the TOF-SIMS

Jean-Paul Barnes¹, Gregory L. Fisher²

1: Univ. Grenoble Alpes, CEA, Leti, F-38000, Grenoble, France; 2: Physical Electronics, Chanhassen, Minnesota 55317, United States

Smaller proteins, bigger data

Anna Kotowska

University of Nottingham, United Kingdom

Subcellular spatial distribution of protein turnover in neuronal progenitor cells

Alicia A. Lork¹, Stefania Rabasco¹, Carl Ernst², Silvio O. Rizzoli³, Nhu T.N. Phan¹

1: Department of Chemistry and Molecular Biology, University of Gothenburg, Sweden; 2: Human Genetics, McGill University, H4H1R3 Montreal, Canada; 3: Department of Neuro- and Sensory Physiology, University Medical Center Göttingen; Center for Biostructural Imaging of Neurodegeneration, Göttingen, Germany

Impact of polymer film structure on interactions with peptides, proteins and bacteria strains

Kamil Awsiuk¹, Yurij Stetsyshyn², Andrzej Budkowski¹, Joanna Raczkowska¹, Natalia Janiszewska¹

1: M. Smoluchowski Institute of Physics, Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University; 2: Lviv Polytechnic National University

Targeted molecular imaging with correlative NanoSIMS: Applications to study protein organization and turnover in neuronal cells

Stefania Rabasco¹, Selda Kabatas², Paola Agüi-Gonzalez^2,3, Alicia Andrea Lork¹, Emmanuel Berlin¹, Felipe Opazo³, Silvio O. Rizzoli^2,3, Nhu T.N. Phan¹

1: Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden; 2: Center for Biostructural Imaging of Neurodegeneration, University Medical Center Göttingen, Göttingen, Germany; 3: Department of Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany

An Anthology of Integrative ToF-SIMS Imaging

Sebastiaan Van Nuffel^1,2

1: M4i, Maastricht University, The Netherlands; 2: Faculty of Science and Engineering, Maastricht University, The Netherlands

Using OrbiSIMS as an Alzheimer’s disease screening tool to investigate ApoE4-mediated molecular alteration of H4 neuroglioma cells

Li {Jennifer} Lu¹, Anna M. Kotowska¹, Stefanie Kern¹, Min Fang², Timothy R Rudd², Morgan R. Alexander¹, David J. Scurr¹, Zheying Zhu¹

1: School of Pharmacy, The University of Nottingham, University Park Campus, NG7 2RD, UK; 2: National Institute for Biological Standards and Control (NIBSC), Blanche Lane, South Mimms, EN6 3QG UK

ToF-SIMS as a matured asset in the plant physiology toolbox? Tracking down salt tolerance mechanisms in rose leaves.

Arne Krause¹, Stefanie Janine Jung², Volker Wissemann², Anja Henss¹

1: Institute of Physical Chemistry and Centre for Material Research, Justus-Liebig-Universität Gießen, Germany; 2: Institute of Systematic Botany, Justus Liebig University Giessen, Germany

ORBITRAP-SIMS IMAGING REVEALS CELL-TYPE SPECIFIC LOCALIZATION OF TOMATO SECONDARY METABOLITES AND ALLOWS PUTATIVE METABOLITE ANNOTATION

Uwe Heinig¹, Julia Zakel², Alexander Pirkl², Yonghui Dong¹, Matthias Kleine-Boymann², Asaph Aharoni¹

1: Weizmann Institute of Science, Rehovot, Israel; 2: IONTOF GmbH, Münster, Germany

Evaluating the depth distribution of organics within porous oxide nanostructures - FIB'n'SIMS vs. cross section mapping vs. depth profiles of hybrid nanoarchitectures

Patrick Hartwich, Gabriel Onyenso, Christian Pritzel, Swathi Naidu Vakamulla Raghu, Manuela S. Killian

Chemistry and Structure of novel Materials, University of Siegen, Germany

High-resolution FIB-STIM-SIMS imaging of bio samples under cryogenic conditions

Antje Kristina Biesemeier, Tatjana Taubitz, Olivier De Castro, Tom Wirtz

Advanced Instrumentation for Nano-Analytics, Materials Research and Technology Department, Luxembourg Institute of Science and Technology, Belvaux, Luxembourg

In-situ interface investigations with FIB-SIMS platform

Pablo Maria Delfino^1,2, Guillaume Lamblin³, Tom Wirtz¹, Jean-Nicolas Audinot¹

1: Advanced Instrumentation for Nano-Analytics (AINA), MRT Department, Luxembourg Institute of Science and Technology (LIST), Belvaux, Luxembourg; 2: University of Luxembourg, 4365 Esch-sur-Alzette, Luxembourg; 3: Transparent and Optically Tuneable, MRT Department, Luxembourg Institute of Science and Technology (LIST), Belvaux, Luxembourg