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Session Chair: Sarah Preston, Federation University Australia
Location:Plenary Lecture Theatre 1, Uni of Auckland Engineering Complex Bldg, LT 405-470
Lecture Theatre 405-470
IJP:DDR Lecturer (Sponsored by Elsevier) Dr Petra Matoušková, Charles University, Prague, Czech Republic
Presentations
Circulation of anthelmintics in the environment enhances anthelmintics deactivation in parasitic nematodes
Petra Matoušková, Martina Navrátilová, Diana Dimunová, Lenka Skálová
Faculty of Pharmacy, Charles University, Czech Republic
Veterinary anthelmintics excreted from treated ruminants enter the environment, can be absorbed by plants, and, upon grazing, re-enter the ruminants 1. Such circulation of anthelmintics can influence non-target species, particularly free-living invertebrates. However, contact with traces of anthelmintics can also promote drug resistance in helminths. Our research focuses on the non-target site mechanisms of drug resistance in nematodes, which can be based on the enhanced deactivation of anthelmintics via their increased biotransformation and elimination from the nematode body. In previous studies, increased expression and activity of xenobiotic-metabolizing enzymes (XME) in drug-resistant strains of Haemonchus contortus were found. In addition, significantly faster deactivation of benzimidazole anthelmintics via oxidation or reduction and subsequent glucose conjugation was detected in resistant strains in comparison to susceptible one. We have explored the effect of sub-lethal concentrations of anthelmintics on H. contortus adults in vitro and in vivo, mimicking the real farm conditions and exploring their effect on the expression and activity of various XME2. Alarmingly, even the lowest concentration of anthelmintics caused enhancement of biotransformation in H. contortus, which can potentially result in inefficient treatment of haemonchosis.
This project was supported by New Technologies for Translational Research in Pharmaceutical Sciences /NETPHARM, project ID CZ.02.01.01/00/22_008/0004607.
1 Navrátilová et al. (2021) Proof of the environmental circulation of veterinary drug albendazole in real farm conditions. Environmental Pollution 286: 117590
2 Dimunová et al. (2022) Environmental circulation of the anthelmintic drug albendazole affects expression and activity of resistance-related genes in the parasitic nematode Haemonchus contortus. Science of the Total Environment 822: 153527
