11:30am - 11:45amTH2-4: 1
Relating Respiratory Aerosol Emission Rates, the Exhaled Carbon Dioxide Flux and the Airborne Survival of Pathogens to Assess Transmission Risk in Indoor Environments
Jonathan P. Reid1, Bryan Bzdek1, Allen Haddrell1, Nan Zhou1, Andrew Shrimpton2, Tony Pickering2, Andrew Davidson3, Darryl Hill3, Adam Finn3, tristan Cogan4, Jamie Mann4
1School of Chemistry, University of Bristol, UK; 2School of Physiology, Pharmacology & Neuroscience, University of Bristol, UK; 3School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK; 4Bristol Veterinary School, University of Bristol, Langford House, Langford, Bristol, UK
Quantifying exhaled aerosol and factors that govern the airborne survival of pathogens are crucial steps in improving our understanding of airborne disease transmission. We will present data from a longitudinal study of the amount of aerosol exhaled by individuals and the correlation with exhaled carbon dioxide. We will show that carbon dioxide in an indoor environment does not necessarily reflect the amount of airborne aerosol and pathogen. In addition, we will show the level of carbon dioxide can impact on the survival of airborne pathogens. Both factors must be considered when implementing effective mitigations to reduce airborne viral transmission.
11:45am - 12:00pmTH2-4: 2
Evaluating Collection Efficiency of a Membrane-based Sampler for Environmental DNA and Bacillus globigii Spores
Gozde Isik1, Loic Coudron1, Ian Johnston1, Richard Kaye1, Chris Stopford1, Christabel Tan1, Shan Goh2, Lanka Weeasiri1, Nikolay Dimov1
1Wolfson Centre for Bio detection Instrumentation Research (WCBIR), School of Physics, Engineering and Computer Science, University of Hertfordshire; 2School of Life and Medical Science, University of Hertfordshire
This research investigated the collection efficiency of small bioaerosols which is Bacillus globigii (B.G.) and environmental DNA (eDNA) of B.G using a novel, wearable membrane-based aerosol sampling device called the Compact Personal Aerosol Sampler (CPAS). Aerosolised bacterial spores and eDNA were collected inside a miniature chamber for the capture, recovery, and quantification of DNA.
12:00pm - 12:15pmTH2-4: 3
Aerosolization triggers de novo synthesis of ice nucleating proteins in the plant pathogen Pseudomonas syringae
Corina Wieber1,2,3, María Palomeque Sánchez1, Sigurd Christiansen4, Meilee Ling5, Augusta Kjelstrup Isaksen1, Merete Bilde2,3,6,7, Thomas Boesen7,8,9,10, Bernadette Rosati3,11, Kai Finster1,2,6, Tina Šantl-Temkiv1,2,6,12
1Department of Biology, Section for Microbiology, Aarhus University, Aarhus, 8000, Denmark; 2iCLIMATE, Interdisciplinary Centre for Climate Change, Aarhus University, Roskilde, 4000, Denmark; 3Department of Chemistry, Aarhus University, Aarhus, 8000, Denmark; 4Faculty of Science and Technology, University of the Faroe Islands, Tórshavn, 100, Faroe Islands; 5FMC Corporation, Hørsholm, 2970, Denmark; 6Arctic Research Centre, Aarhus University, Aarhus, 8000, Denmark; 7iNANO, Interdisciplinary Nanoscience Center, Aarhus University, Aarhus, 8000, Denmark; 8Department of Molecular Biology and Genetics, Protein Science, Aarhus University, Aarhus, 8000, Denmark; 9Department of Biology, Center for Electromicrobiology, Aarhus University, Aarhus, 8000, Denmark; 10DANDRITE, Aarhus University, Aarhus, 8000, Denmark; 11University of Natural Resources and Life Sciences, Vienna, 1180, Austria; 12Stellar Astrophysics Centre, Aarhus University, Aarhus, 8000, Denmark
Pseudomonas syringae is a common plant pathogen, posing a significant threat to the global crop production. Some strains of P. syrinage can cause frost injuries on plants and influence cloud properties and precipitation through ice nucleating proteins (INpro). The study investigated the mechanisms driving aerosolization and INpro production in the model organism P. syringae R10.79. Aerosolization by bubble-bursting resulted in a higher fraction of INpro-bearing cells after aerosolization (33.2%) compared to before (10.7%). A significant positive correlation between the fraction of viable cells and the increase in INpro-bearing cells after aerosolization indicates that INpro are synthesized de novo.
12:15pm - 12:30pmTH2-4: 4
Characterization of Bioaerosols Using Mass Spectrometric Techniques
Rawan Ajaj, Yufang Hao, Abdul Aziz Kurdieh, Claudia Mohr, André Prévôt, Kaspar Rudolf Daellenbach, Lubna Dada
Paul Scherrer Institute, Switzerland
Bioaerosols, including fungal spores, bacteria, and pollen, impact air quality, health, and climate but are difficult to characterize. To address this, we developed a mass spectrometry-based method combining AMS for bulk composition and EESI-Orbitrap-MS for molecular identification. Laboratory experiments confirmed its ability to distinguish bioaerosols, leading to the development of a mass-spectral library to support field measurements in Switzerland for tracing emission sources and seasonal trends.
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