11:30am - 11:45amWE2-2: 1
Toxicological effect of fresh and aged aerosol modified in an environment simulation chamber in an alveolar model exposed at the air liquid interface
Sara Marchetti1, Virginia Vernocchi2, Giulia Motta1, Gianluigi De Falco3, Mario Commodo3, Andrea D'Anna5, Tommaso Isolabella2,4, Dario Massabò2,4, Patrizia Minutolo3, Paolo Prati2,4, Maurizio Gualtieri1
1Department of Environmental and Earth Sciences, University of Milano-Bicocca, Research Centre POLARIS Piazza della Scienza 1, Milano Italy; 2INFN Sezione di Genova, Via Dodecaneso 33, 16146 Genova Italy; 3Istituto di Scienze e Tecnologie per l'Energia e la Mobilità Sostenibili, STEMS-CNR, P.le Tecchio 80, 80125 - Napoli (Italy); 4Department of Physics, University of Genova, Via Dodecaneso 33, 16146 Genova Italy; 5DICMAPI, Università degli Studi di Napoli Federico II, P.le Tecchio 80, Napoli 80125, Italy
Despite the extensive knowledge on the toxicological effects of PM2.5 several grey areas remain. Among these, the understanding of the different toxicological effects of fresh versus aged particles remains poorly understood. Here we present the results obtained by coupling an online exposure module (Cultex RFS Compact) with an atmospheric simulation chamber (ChaMBRe). Ultrafine particles were produced by a lab scale laminar premixed flame using ethylene and a mixture of ethylene and ethanol. The results reported showed the importance of understanding the impact of aging processes on the toxicological properties of soot particles produced from both conventional fossil fuels and biofuels.
11:45am - 12:00pmWE2-2: 2
Adverse effects of fresh and aged high-power engine emissions on lung tissue models at the Air-Liquid Interface
Sebastiano Di Bucchianico1, Mohammad Jamil Almasaleekh1, Johannes Becker1, Wesam Darwish1, Mika Ihalainen2, Uwe Etzien1, Thorsten Streibel1, Olli Sippula2, Bert Buchholz1, Ralf Zimmermann1
1University of Rostock, Germany; 2University of Eastern Finland, Finland
This study examined the toxicological effects of exhaust emissions from high-power engines, including JP-8 jet fuel combustion and ship diesel emissions (MGO and HFO). We assessed on lung cell models the effects of fresh and photochemically aged emissions at the Air-Liquid Interface. Fresh aerosols primarily induced oxidative stress, while aged emissions triggered stronger pro-inflammatory responses. DNA damage varied by fuel type and aging state. Despite atmospheric aging significantly altering emissions' properties, its impact on toxicity remains inconclusive. The study highlights the importance of exposure metrics (mass, particle number, surface area) and chemical composition in assessing health risks of ultrafine particles.
12:00pm - 12:15pmWE2-2: 3
Assessing Exhaust Gas Exposure in Real Driving Conditions with a Portable Air-Liquid Interface Chamber
Michal Vojtisek-Lom1,2, Lubos Dittrich2, Tereza Cervena1, Katerina Honkova1, Tana Zavodna1, Martin Pechout3, Pavel Rössner1, Anssi Järvinen4, Hannu Kuutti4, Petteri Marjanen5, Teemu Lepistö5, Laura Salo5, Katarina Kylämäki5, Milja Jäppi5, Wojciech Honkisz6, Piotr Bielaczyc6, Andrzej Szczotka6, Hilkka Timonen7, Henna Lintusaari5, Leila Simon7, Sanna Saarikoski7, Topi Rönkkö5, Päivi Aakko-Saksa4, Jan Topinka1
1Institute of Experimental Medicine of the Czech Academy of Sciences, Prague, Czech Republic; 2Technical University of Liberec, Liberec, Czech Republic; 3Czech University of Life Sciences, Prague, Czech Republic; 4VTT Technical Research Centre of Finland Ltd, Espoo, Finland; 5Aerosol Physics Laboratory, Physics Unit, Tampere University, Tampere, Finland; 6BOSMAL Automotive Research and Development Institute Ltd, Bielsko-Biala, Poland; 7Finnish Meteorological Institute, Helsinki, Finland
This work combines real-world, on-road emissions monitoring with toxicity assays using exposure of cell cultures at air-liquid interface. Cell cultures on 6 mm inserts are placed in a small airtight box, mounted in a portable incubator placed in the tested vehicle and fed with diluted engine exhaust conditioned to 5% CO2, 37°C and >85% humidity at 25 cm³/min per insert. Deposition rate by diffusion is 1.5% for 10 and 21 nm particles. Successful field exposures of 1-20x 1-2 hours at -5 to +32 C with exhaust, outdoor air, nanoparticles. Funding: HE PAREMPI
12:15pm - 12:30pmWE2-2: 4
In vitro aerosol exposure systems for respiratory health monitoring: strategies for optimizing deposition efficacy
Magdalena Weiß1,2, Benjamin Punz1, Evelien Frijns3, Sandra Verstraelen3, Cristian-Tudor Matea1, Melanie Schürz1, Nicole Meisner-Kober1, Otmar Schmid2, Martin Himly1
1Dept. Biosciences & Med. Biology, Paris Lodron University Salzburg (PLUS), Hellbrunnerstrasse 34, 5020 Salzburg, Austria; 2Lung Health and Immunity, Helmholtz Munich, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany; 3Environmental Intelligence Unit, Flemish Institute for Technological Research (VITO), Boeretang 200, 2400 Mol, Belgium
Unintentional lung exposure to air pollution and airborne pathogens is omnipresent. This study presents NAVETTA, an in vitro aerosol exposure system that replicates lung deposition. It uses an inverted Transwell setup within an electric field to enhance particle deposition from a low laminar airflow. NAVETTA ensures uniform deposition (CV <15%) across four positions and supports nanovesicle drug delivery studies. Its performance aligns with existing models, including the radially symmetric stagnation point flow system. NAVETTA is a reliable tool for drug delivery and risk assessment research, aiding lung disease studies while reducing the need for animal testing.
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