10:15am - 10:30amWE1-2: 1
Toxicological effects of laboratory-generated SOA from the day- and nighttime oxidation of PAHs and phenol on an enhanced alveolar epithelium model exposed at the air-liquid interface (ALI)
Aurélia Alunni1, Maxime Floreani1, Guillaume Barbier1, Céline Ferret1, Adrien Dermigny1, Rachel Gemayel1, Jérôme Beaumont1, Ahmad El-Masri1, Khristy Pinto1, Martin Figeac2, Guillaume Garçon3, Bénédicte Trouiller1, Alexandre Albinet1
1INERIS, Parc Technologique Alata, Verneuil en Halatte, 60550, France; 2Lille University, Lille Hospital, CNRS, INSERM, Institut Pasteur de Lille, US 41 - UAR 2014 - PLBS, 59000, Lille, France; 3Lille Neurosciences & Cognition - UMRS 1172, Lille University, Lille, 59000, France
Residential wood combustion (RWC) represents a significant source of volatile organic compounds leading to secondary organic aerosol (SOA) formation. The pulmonary toxicity of SOA formed from key precursors emitted by RWC remains poorly understood. This study aims to assess the toxicological effects of laboratory-generated SOA from day- and nighttime oxidation of PAHs and phenol on an alveolar epithelium model exposed at the air-liquid interface. Biological responses including metabolic activity, inflammation, vesicles exocytosis, genotoxicity, and gene expression have been studied. Results will contribute to understand the modulation of specific cellular mechanisms involving different cell signaling pathways depending on the SOA considered.
10:30am - 10:45amWE1-2: 2
Flavoured electronic cigarette aerosols induce transcriptomic and metabolomic changes to alveolar epithelial cells exposed at the air-liquid interface
Mathilde Noemie Delaval1, Rasha Alsaleh2, Christian Kersch2, Carmen Adrian2, Jan Heide3, Selina Rinaldi4, Sven Ehlert3, Elke Pieper4, Simone Schmitz-Spanke2, Ralf Zimmermann1,3
1Helmholtz Munich, Germany; 2University of Erlanger-Nuremberg, Germany; 3University of Rostock, Germany; 4German Federal Institute for Risk Assessment, Germany
This study investigates the role of flavors in the toxicity of electronic cigarette (e-cig) aerosols using an in vitro alveolar model. A549 cells at the air-liquid interface were exposed to unflavored and flavored (vanillin, cinnamaldehyde, eugenol) e-cig aerosols generated under controlled conditions. Toxicological assessments revealed minor cytotoxic effects, increased IL-6 release with cinnamaldehyde, and transcriptomic and metabolomic alterations, possibly linked to xenobiotic metabolism. Eugenol exposure led to significant metabolite downregulation. While short-term exposure induced limited toxicity, flavors influenced biological responses, highlighting the need for further research on their contribution to e-cig-related health risks.
10:45am - 11:00amWE1-2: 3
Exposure to PM oxidative potential and inflammatory biomarkers in vulnerable populations: the ASTHMA-FENOP and PEREX-COPD studies
Ignacio Fernández-Olmo1, Andrea Expósito1, Juan Agüero-Calvo2, Juan Luis García-Rivero2, Beatriz Abascal2, Carlos Antonio Amado2, Marcos López-Hoyos3, Miguel Santibáñez4
1Departamento de Ingenierías Química y Biomolecular, Universidad de Cantabria, Spain; 2Division of Pneumology, Hospital Universitario Marqués de Valdecilla, IDIVAL; 3Division of Immunology, Hospital Universitario Marqués de Valdecilla, IDIVAL; 4Global Health Research Group, Faculty of Nursing, Universidad de Cantabria; IDIVAL, Spain
This work aims to study the association between PM oxidative potential (OP) exposure and inflammatory biomarkers in vulnerable populations in Santander (northern Spain): patients with asthma and chronic obstructive pulmonary disease (COPD). To this end, the ASTHMA-FENOP and PEREX-COPD studies were designed; PM OP (OP-AA and OP-DTT) exposure was determined in the fine and coarse fractions of PM samples collected by personal samplers, and respiratory and systemic inflammatory biomarkers were measured in the exposed volunteers (unhealthy and healthy groups). The strongest association between increased PM OP exposure and inflammation was found for the OP-DTT assay measured in the fine fraction.
11:00am - 11:15amWE1-2: 4
Inhalable carbon fibres – CF aerosol generation, characterisation and air-liquid interface exposure
Sonja Mülhopt1, Manuela Hauser1, Manuela Wexler1, Jonathan Mahl1, Werner Baumann1, Sven Klein2, Silvia Diabaté2, Susanne Fritsch-Decker2, Carsten Weiss2, Alexandra Friesen3, Matthias Hufnagel3, Andrea Hartwig3, Bastian Gutmann4, Christoph Schlager4, Tobias Krebs4, Dieter Stapf1
1Institute for Technical Chemistry, Karlsruhe Institute of Technology , Germany; 2Institute for Biological and Chemical Systems - Biological Information Processing, Karlsruhe Institute of Technology, Germany; 3Institute for Applied Biosciences, Karlsruhe Institute of Technology, Germany; 4Vitrocell Systems GmbH, Germany
Carbon fibres and CF-reinforced plastics are innovative materials, which are increasingly produced, recycled, and disposed of, possibly releasing fibres which could fulfil the criteria of the World Health Organisation (WHO) to be potentially carcinogenic (critical aspect ratio > 3:1, length ≥ 5 μm and diameter ≤ 3μm). Carbon fibres were dispersed into dry air and delivered to the air-liquid interface (ALI) of human lung cells, where toxicological investigations were carried out. The deposition behaviour of the three fractions of the carbon fibre aerosol was measured on the one hand and simulated by numerical methods on the other.
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