Conference Agenda

Overview and details of the sessions of this conference. Please select a date or location to show only sessions at that day or location. Please select a single session for detailed view (with abstracts and downloads if available).

 
 
Session Overview
Date: Monday, 28/Sept/2020
10:00am
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10:05am
Welcome to Satellite Webinars
Location: Zoom

Welcome by the president of the DGfZ: Prof. Anja Hauser

10:05am
-
10:40am
Satellite Webinar 1: Becton Dickinson (BD)
Chair: Anja Hauser

Introduction by chair and Becton Dickinson followed by a 30 min webinar

Date: Tuesday, 29/Sept/2020
10:00am
-
10:35am
Satellite Webinar 2: OMNI Life Science GmbH & Co. KG (OLS)
Location: Zoom
Chair: Christin Koch

Introduction by chair and OMNI Life Science followed by a 30 min webinar

10:35am
-
11:00am
Break
Location: Zoom
11:00am
-
11:35am
Satellite Webinar 3: APE
Location: Zoom
Chair: Asylkhan Rakhymzhan

Introduction by chair and APE followed by a 30 min webinar

Date: Wednesday, 30/Sept/2020
10:00am
-
10:35am
Satellite Webinar 4: Propel Labs
Location: Zoom
Chair: Wolfgang Fritzsche

Introduction by chair and Propel Labs followed by a 30 min webinar

10:35am
-
11:00am
Break
Location: Zoom
11:00am
-
11:35am
Satellite Webinar 5: Luminex
Location: Zoom
Chair: Frank Schildberg

Introduction by chair and Luminiex followed by a 30 min webinar

Date: Thursday, 01/Oct/2020
9:00am
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9:05am
Welcome 2: Welcome to DiGifZ 2020
Location: Zoom

Welcome to DiGifZ 2020 by the president of the German Society of Cytometry (DGfZ), Prof. Anja Hauser

9:05am
-
10:00am
Session 1: Jubilee Lecture: 30 Years DGfZ: A personal view
Location: Zoom
Chair: Andreas Radbruch
Chair: Elmar Endl

Introduction by Andreas Radbruch

Lecture by Günter Valet (Founding member of the DGfZ)

 

30 years DGfZ: A personal view

Günter Valet

Max-Planck-Institut für Biochemie, Martinsried (retired), Germany

10:00am
-
10:15am
Break
Location: Zoom
10:15am
-
10:50am
Satellite Webinar 6: Fluidigm
Location: Zoom
Chair: Henrik Mei

Introduction by chair and Fluidigm followed by a 30 min webinar

10:50am
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11:15am
Break
Location: Zoom
11:15am
-
12:15pm
Session 2: Keynote Lecture
Location: Zoom
Chair: Anja Hauser

Introduction by Anja Hauser

Lecture by Michael Dustin (Kennedy Institute of Rheumatology, UK)

 

Use of multiparameter flow cytometry to quantify the output of the immunological synapse

Michael Dustin

Kennedy Institute of Rheumatology, United Kingdom

12:15pm
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12:30pm
Break
Location: Zoom
12:30pm
-
1:30pm
Session 3: Poster Session
Location: Zoom
Chair: Attila Tarnok
Chair: Torsten Viergutz

Session Chair: Raluca Niesner

Please submit abstracts and posters for virtual poster session

 

A comprehensive analysis of intestinal microbiota by multi-parameter flow cytometry

Lisa Budzinski, Toni Sempert, René Maier, Alexander Beller, Gitta A Heinz, Ute Hoffmann, Pawel Durek, Mir-Farzin Mashreghi, Hyun-Dong Chang

German Rheumatism Research Centre Berlin - A Leibniz Insitute, Germany



Characterization of chemical-specific CD4+ T cells in human blood

Caterina Curato1,2, Marina Aparicio-Soto1,2, Franziska Riedel1,2, Ingrun Wehl1,2, Alev Basaran1,2, Melanie Leddermann1,2, Uwe Hillen3, Hermann-Josef Thierse2, Andreas Luch1,2, Katherina Siewert1,2

1: Allergy Study Centre, German Federal Institute for Risk Assessment, Berlin, Germany; 2: Department of Chemical and Product Safety, German Federal Institute for Risk Assessment, Berlin, Germany; 3: Clinics of Dermatology and Venerology, Vivantes Klinikum Neukölln, Berlin, Germany



Deeper insights in neutrophil metabolism by systematic enzyme mapping based on phasor-analyzed label-free NAD(P)H-FLIM

Ruth Leben1, Markus Köhler1, Helena Radbruch2, Anja E. Hauser3, Raluca A. Niesner1,4

1: Biophysical Analytics, German Rheumatism Research Center (DRFZ) , Berlin; 2: Institute for Neuropathology, Charité – Universitätsmedizin, Berlin; 3: Immune Dynamics, German Rheumatism Research Center (DRFZ), Berlin; 4: Dynamic and Functional in vivo Imaging, Free University, Berlin



High-throughput cell and spheroid mechanics in virtual fluidic channels

Muzaffar H. Panhwar1, Fabian Czerwinski1, Venkata A. S. Dabbiru1, Yesaswini Komaragiri1, Bob Fregin1, Doreen Biedenweg2, Peter Nestler1, Ricardo H. Pires1, Oliver Otto1

1: ZIK HIKE, University of Greifswald, Greifswald, Germany; 2: University Medicine, Greifswald



Integrated Cytomics platform for improved environmental monitoring of pollination, air and water quality, combining high throughput (imaging) cytometry and deep learning

Susanne Dunker1,2

1: Helmholtz-Centre for Environmental Research - UFZ, Germany; 2: German Centre for Integrative Biodiversity Research - iDiv, Germany



Light scattering pulse shape analysis with multiple forward scatter detectors in flow cytometry

Daniel Kage1, Kerstin Heinrich1, Konrad v. Volkmann2, Jenny Kirsch1, Claudia Giesecke-Thiel3, Toralf Kaiser1

1: German Rheumatism Research Centre Berlin (DRFZ) - Flow Cytometry Core Facility; 2: APE Angewandte Physik und Elektronik GmbH; 3: Max Planck Institute for Molecular Genetics (MPIMG) - Flow Cytometry Facility



Limbostomy: Longitudinal Intravital Microendoscopy in Murine Osteotomies

Alexander Ferdinand Fiedler1,2, Jonathan Stefanowski3,4, Markus Köhler2,3, Robert Günther1, Wjatscheslaw Liublin1, Martin Tschaikner1, Ariana Rauch3, David Reismann1, Romano Matthys5, Reto Nützi5, Gabriele Bixel6, Ralf H. Adams6, Georg N. Duda7, Raluca A. Niesner1,2, Anja E. Hauser3,4

1: Biophysical Analytics, German Rheumatism Research Center (DRFZ), Berlin, Germany; 2: Veterinary Medicine, Freie Universität Berlin, Berlin, Germany; 3: Immune Dynamics, German Rheumatism Research Center (DRFZ), Berlin, Germany; 4: Department of Rheumatology and Clinical Immunology, Charité – Universitätsmedizin, Berlin, Germany; 5: RISystem AG, RISystem AG, Landquart, Switzerland; 6: Tissue Morphogenesis, Max Planck Institute for Molecular Biomedicine, Münster, Germany; 7: Julius Wolff Institute, Charité – Universitätsmedizin, Berlin, Germany



Longitudinal Intravital Multiphoton Microendoscopy Reveals Vascularization during Bone Regeneration To Occur in Two Distinct Phases and To Be Preceded by CX3CR1+ Myeloid Cells

Jonathan Stefanowski1,2, Alexander F. Fiedler1,4, Ariana Rauch1, Annemarie Lang1,2,3, Raluca A. Niesner1,4, Anja E. Hauser1,2

1: Deutsches Rheuma-Forschungszentrum, 10117 Berlin, Germany; 2: Department of Rheumatology and Clinical Immunology, Charité - Universitätsmedizin, 10117 Berlin, Germany; 3: Berlin-Brandenburg Center for Regenerative Therapies, Charité - Universitätsmedizin, 10117 Berlin, Germany; 4: Dynamic and Functional in vivo imaging, Department of Veterinary Medicine, Freie Universität Berlin, Germany



ROS induced cell mechanical alterations in suspension and adherent cells

Yesaswini Komaragiri1, Huy Tung Dau1, Doreen Biedenweg2, Ricardo Pires1, Oliver Otto1

1: Biomechanics, ZIK-HIKE, Universität Greifswald, Greifswald, Germany; 2: Universitätsmedizin Greifswald, Greifswald, Germany



SARS-Cov-2 induces enhanced intestinal immune responses in severe COVID-19 patients

Justus Ninnemann1, Lisa Budzinski1, Gitta Heinz1, Marta Ferreira-Gomes1, Caroline Tizian2, Stefan Angermair2, Sascha Treskatsch2, Thomas Dörner2, Hyun-Dong Chang1, Mario Witkowski2, Andreas Radbruch1, Mir-Farzin Mashreghi1, Andrey Kruglov1

1: German Rheumatism Research Center (DRFZ), a Leibniz Institute, Berlin 10117, Germany; 2: Charité-Universitätsmedizin Berlin



Spatially resolved, in-depth cell profiling and quantification in human tissues by Multiplexed Histology and its biological applications

Anna Pascual Reguant1,2, Ronja Mothes1,2, Sandy Bauherr2, Ralf Köhler2,3, Daniela Hernandez2, Theresa Dornieden1, Arne Sattler1, Katja Kotsch1, Chiara Romagnani1,2, Raluca Niesner2,3, Helena Radbruch1, Anja Hauser1,2

1: Charité Universitätsmedizin Berlin, Germany; 2: DRFZ Berlin, Germany; 3: Freie Universität Berlin, Germany



Stress-strain relation in virtual fluidic channels analyzed by particle image velocimetry

Peter Nestler, Muzaffar H. Panhwar, Oliver Otto

University Greifswald, Germany



Superposition of viscosity maps and metabolism in parasitically infested intestinal tissue using BODIPY and NAD(P)H fluorecence lifetimes

Wjatscheslaw Liublin1, Ruth Leben1, Robert Günther2, Jonathan Stefanowski3, Juliane Liebeskind3, Ingeborg Beckers4, Ingo Röhle4, Alina Liebheit3, Carolin Ulbricht3, Alexander F. Fiedler1, Anja E. Hauser3, Sebastian Rausch5, Susanne Hartmann5, Raluca Niesner1

1: Biophysical Analytics, DRFZ - A Leibniz Institute, Berlin; Dynamic and functional in vivo imaging, Veterinary Medicine, Freie Universität, Berlin; 2: DRFZ - A Leibniz Institute, Berlin; 3: Immunodynamics, DRFZ - A Leibniz Institute, Berlin; Immunodynamics and intravital microscopy, Charité, Berlin; 4: Beuth School for Applied Sciences; 5: Institute for Immunology, Veterinary Medicine, Freie Universität, Berlin



Testing the B cell surfaceome by a bar-coded cytometry assay

Michael Reth, Yaneth Ortiz, Kathrin Kläsener, Niklas Vesper, Jianying Yang

Albert-Ludwigs-Universität Freiburg, Germany



The role of surface contacts in 2D and 3D microenvironments for cell mechanical properties

Venkata Aditya Saimadhukiran Dabbiru1, Emmanual Manu1, Huy Tung Dau1, Nora Bödecker1, Doreen Biedenweg2, Ricardo H Pires1, Oliver Otto1

1: ZIK HIKE, University of Greifswald, Germany; 2: University Medicine Greifswald, Germany

1:30pm
-
2:30pm
Break
Location: Zoom
2:30pm
-
3:05pm
Satellite Webinar 7: Sony
Location: Zoom
Chair: Asylkhan Rakhymzhan

Introduction by chair and Sony followed by a 30 min webinar.

3:05pm
-
3:30pm
Break
Location: Zoom
3:30pm
-
4:30pm
Session 4: Core Facility Session: Spectral Cytometry
Location: Zoom
Chair: Frank Schildberg
Chair: Christian Kukat

Speakers: Simone Pöschel (Tübingen) and Bastian Höchst (München)

 

Implementing spectral cytometry in a core facility: one lab's experience with the Cytek Aurora

Simone Pöschel

Universitätsklinikum Tübingen, Germany



The metabolic momentum of MDSCs

Bastian Höchst

Technische Universität München, Germany

4:30pm
-
6:30pm
Core Facility Networking Event
Location: Zoom
Chair: Desiree Kunkel
Chair: Christian Kukat

Almost the same procedure as last year… This year we will meet, drink, eat and network in virtual space!

For more information, please see here:

https://digifz2020.de/the-core-facility-networking-event/

Date: Friday, 02/Oct/2020
9:00am
-
9:35am
Satellite Webinar 8: Beckman Coulter
Location: Zoom
Chair: Anja Hauser

Introduction by chair and Beckman Coulter followed by a 30 min webinar.

9:35am
-
10:00am
Break
Location: Zoom
10:00am
-
10:50am
Session 5: Cutting Edge (Sars-CoV-2) Part 1
Location: Zoom
Chair: Raluca Niesner
Chair: Anja Hauser

Invited speaker: Andrea Cossarizza (ISAC president, University of Modena)

 

Immune changes during COVID-19 pneumonia

Andrea Cossarizza

University of Modena and Reggio Emilia School of Medicine, Italy

10:50am
-
11:50am
Session 6: Product Slam
Location: Zoom
Chair: Elmar Endl
Chair: Thomas Bauer

Selected industrial partners will present their newest innovative technological developments and products

11:50am
-
1:00pm
Break
Location: Zoom
1:00pm
-
2:00pm
Session 7: Cutting Edge (Sars-CoV-2) Part 2
Location: Zoom
Chair: Attila Tarnok
Chair: Asylkhan Rakhymzhan

Combined approaches for COVID-19 Cytometry in Berlin

Speakers: Leif Erik Sander (Charité), Helena Radbruch (Charité) and Mir-Farzin Mashreghi (DRFZ and BCRT)  will present data from their current Covid-19 studies

 

Dissecting innate immune responses to SARS-CoV-2

Leif Erik Sander

Charité Universitätsmedinzin Berlin, Germany



Olfactory transmucosal SARS-CoV-2 invasion as port of Central Nervous System entry in COVID-19 patients

Helena Radbruch

Charité Universitätsmedizin Berlin, Germany



In severe COVID-19, SARS-CoV-2 induces a chronic, TGF-β-dominated adaptive immune response

Mir-Farzin Mashreghi

DRFZ, Germany

2:00pm
-
2:20pm
Break
Location: Zoom
2:20pm
-
2:55pm
Satellite Webinar 9: Miltenyi
Location: Zoom
Chair: Elmar Endl
Chair: Thomas Bauer

Introduction by chair and Miltenyi followed by a 30 min webinar.

2:55pm
-
3:15pm
Break
Location: Zoom
3:15pm
-
3:45pm
Summary, award ceremony and farewell
Location: Zoom
Chair: Anja Hauser

Summary of the online conference

Award ceremony: Poster Prize and Webinar Competition 

Farewell

4:00pm
-
5:00pm
Annual members assembly of the DGFZ
Location: Zoom
Chair: Anja Hauser
Chair: Raluca Niesner

All members receive the program and the link for the zoom meeting by email!


 
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