Conference Agenda

30 years DGfZ: A personal view

Günter Valet

Max-Planck-Institut für Biochemie, Martinsried (retired), Germany

Use of multiparameter flow cytometry to quantify the output of the immunological synapse

Michael Dustin

Kennedy Institute of Rheumatology, United Kingdom

A comprehensive analysis of intestinal microbiota by multi-parameter flow cytometry

Lisa Budzinski, Toni Sempert, René Maier, Alexander Beller, Gitta A Heinz, Ute Hoffmann, Pawel Durek, Mir-Farzin Mashreghi, Hyun-Dong Chang

German Rheumatism Research Centre Berlin - A Leibniz Insitute, Germany

Characterization of chemical-specific CD4+ T cells in human blood

Caterina Curato^1,2, Marina Aparicio-Soto^1,2, Franziska Riedel^1,2, Ingrun Wehl^1,2, Alev Basaran^1,2, Melanie Leddermann^1,2, Uwe Hillen³, Hermann-Josef Thierse², Andreas Luch^1,2, Katherina Siewert^1,2

1: Allergy Study Centre, German Federal Institute for Risk Assessment, Berlin, Germany; 2: Department of Chemical and Product Safety, German Federal Institute for Risk Assessment, Berlin, Germany; 3: Clinics of Dermatology and Venerology, Vivantes Klinikum Neukölln, Berlin, Germany

Deeper insights in neutrophil metabolism by systematic enzyme mapping based on phasor-analyzed label-free NAD(P)H-FLIM

Ruth Leben¹, Markus Köhler¹, Helena Radbruch², Anja E. Hauser³, Raluca A. Niesner^1,4

1: Biophysical Analytics, German Rheumatism Research Center (DRFZ) , Berlin; 2: Institute for Neuropathology, Charité – Universitätsmedizin, Berlin; 3: Immune Dynamics, German Rheumatism Research Center (DRFZ), Berlin; 4: Dynamic and Functional in vivo Imaging, Free University, Berlin

High-throughput cell and spheroid mechanics in virtual fluidic channels

Muzaffar H. Panhwar¹, Fabian Czerwinski¹, Venkata A. S. Dabbiru¹, Yesaswini Komaragiri¹, Bob Fregin¹, Doreen Biedenweg², Peter Nestler¹, Ricardo H. Pires¹, Oliver Otto¹

1: ZIK HIKE, University of Greifswald, Greifswald, Germany; 2: University Medicine, Greifswald

Integrated Cytomics platform for improved environmental monitoring of pollination, air and water quality, combining high throughput (imaging) cytometry and deep learning

Susanne Dunker^1,2

1: Helmholtz-Centre for Environmental Research - UFZ, Germany; 2: German Centre for Integrative Biodiversity Research - iDiv, Germany

Light scattering pulse shape analysis with multiple forward scatter detectors in flow cytometry

Daniel Kage¹, Kerstin Heinrich¹, Konrad v. Volkmann², Jenny Kirsch¹, Claudia Giesecke-Thiel³, Toralf Kaiser¹

1: German Rheumatism Research Centre Berlin (DRFZ) - Flow Cytometry Core Facility; 2: APE Angewandte Physik und Elektronik GmbH; 3: Max Planck Institute for Molecular Genetics (MPIMG) - Flow Cytometry Facility

Limbostomy: Longitudinal Intravital Microendoscopy in Murine Osteotomies

Alexander Ferdinand Fiedler^1,2, Jonathan Stefanowski^3,4, Markus Köhler^2,3, Robert Günther¹, Wjatscheslaw Liublin¹, Martin Tschaikner¹, Ariana Rauch³, David Reismann¹, Romano Matthys⁵, Reto Nützi⁵, Gabriele Bixel⁶, Ralf H. Adams⁶, Georg N. Duda⁷, Raluca A. Niesner^1,2, Anja E. Hauser^3,4

1: Biophysical Analytics, German Rheumatism Research Center (DRFZ), Berlin, Germany; 2: Veterinary Medicine, Freie Universität Berlin, Berlin, Germany; 3: Immune Dynamics, German Rheumatism Research Center (DRFZ), Berlin, Germany; 4: Department of Rheumatology and Clinical Immunology, Charité – Universitätsmedizin, Berlin, Germany; 5: RISystem AG, RISystem AG, Landquart, Switzerland; 6: Tissue Morphogenesis, Max Planck Institute for Molecular Biomedicine, Münster, Germany; 7: Julius Wolff Institute, Charité – Universitätsmedizin, Berlin, Germany

Longitudinal Intravital Multiphoton Microendoscopy Reveals Vascularization during Bone Regeneration To Occur in Two Distinct Phases and To Be Preceded by CX3CR1+ Myeloid Cells

Jonathan Stefanowski^1,2, Alexander F. Fiedler^1,4, Ariana Rauch¹, Annemarie Lang^1,2,3, Raluca A. Niesner^1,4, Anja E. Hauser^1,2

1: Deutsches Rheuma-Forschungszentrum, 10117 Berlin, Germany; 2: Department of Rheumatology and Clinical Immunology, Charité - Universitätsmedizin, 10117 Berlin, Germany; 3: Berlin-Brandenburg Center for Regenerative Therapies, Charité - Universitätsmedizin, 10117 Berlin, Germany; 4: Dynamic and Functional in vivo imaging, Department of Veterinary Medicine, Freie Universität Berlin, Germany

ROS induced cell mechanical alterations in suspension and adherent cells

Yesaswini Komaragiri¹, Huy Tung Dau¹, Doreen Biedenweg², Ricardo Pires¹, Oliver Otto¹

1: Biomechanics, ZIK-HIKE, Universität Greifswald, Greifswald, Germany; 2: Universitätsmedizin Greifswald, Greifswald, Germany

SARS-Cov-2 induces enhanced intestinal immune responses in severe COVID-19 patients

Justus Ninnemann¹, Lisa Budzinski¹, Gitta Heinz¹, Marta Ferreira-Gomes¹, Caroline Tizian², Stefan Angermair², Sascha Treskatsch², Thomas Dörner², Hyun-Dong Chang¹, Mario Witkowski², Andreas Radbruch¹, Mir-Farzin Mashreghi¹, Andrey Kruglov¹

1: German Rheumatism Research Center (DRFZ), a Leibniz Institute, Berlin 10117, Germany; 2: Charité-Universitätsmedizin Berlin

Spatially resolved, in-depth cell profiling and quantification in human tissues by Multiplexed Histology and its biological applications

Anna Pascual Reguant^1,2, Ronja Mothes^1,2, Sandy Bauherr², Ralf Köhler^2,3, Daniela Hernandez², Theresa Dornieden¹, Arne Sattler¹, Katja Kotsch¹, Chiara Romagnani^1,2, Raluca Niesner^2,3, Helena Radbruch¹, Anja Hauser^1,2

1: Charité Universitätsmedizin Berlin, Germany; 2: DRFZ Berlin, Germany; 3: Freie Universität Berlin, Germany

Stress-strain relation in virtual fluidic channels analyzed by particle image velocimetry

Peter Nestler, Muzaffar H. Panhwar, Oliver Otto

University Greifswald, Germany

Superposition of viscosity maps and metabolism in parasitically infested intestinal tissue using BODIPY and NAD(P)H fluorecence lifetimes

Wjatscheslaw Liublin¹, Ruth Leben¹, Robert Günther², Jonathan Stefanowski³, Juliane Liebeskind³, Ingeborg Beckers⁴, Ingo Röhle⁴, Alina Liebheit³, Carolin Ulbricht³, Alexander F. Fiedler¹, Anja E. Hauser³, Sebastian Rausch⁵, Susanne Hartmann⁵, Raluca Niesner¹

1: Biophysical Analytics, DRFZ - A Leibniz Institute, Berlin; Dynamic and functional in vivo imaging, Veterinary Medicine, Freie Universität, Berlin; 2: DRFZ - A Leibniz Institute, Berlin; 3: Immunodynamics, DRFZ - A Leibniz Institute, Berlin; Immunodynamics and intravital microscopy, Charité, Berlin; 4: Beuth School for Applied Sciences; 5: Institute for Immunology, Veterinary Medicine, Freie Universität, Berlin

Testing the B cell surfaceome by a bar-coded cytometry assay

Michael Reth, Yaneth Ortiz, Kathrin Kläsener, Niklas Vesper, Jianying Yang

Albert-Ludwigs-Universität Freiburg, Germany

The role of surface contacts in 2D and 3D microenvironments for cell mechanical properties

Venkata Aditya Saimadhukiran Dabbiru¹, Emmanual Manu¹, Huy Tung Dau¹, Nora Bödecker¹, Doreen Biedenweg², Ricardo H Pires¹, Oliver Otto¹

1: ZIK HIKE, University of Greifswald, Germany; 2: University Medicine Greifswald, Germany

Implementing spectral cytometry in a core facility: one lab's experience with the Cytek Aurora

Simone Pöschel

Universitätsklinikum Tübingen, Germany

The metabolic momentum of MDSCs

Bastian Höchst

Technische Universität München, Germany

Immune changes during COVID-19 pneumonia

Andrea Cossarizza

University of Modena and Reggio Emilia School of Medicine, Italy

Dissecting innate immune responses to SARS-CoV-2

Leif Erik Sander

Charité Universitätsmedinzin Berlin, Germany

Olfactory transmucosal SARS-CoV-2 invasion as port of Central Nervous System entry in COVID-19 patients

Helena Radbruch

Charité Universitätsmedizin Berlin, Germany

In severe COVID-19, SARS-CoV-2 induces a chronic, TGF-β-dominated adaptive immune response

Mir-Farzin Mashreghi

DRFZ, Germany